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Micropropagation of Hippeastrum hybridum ‘Apple Blossom’, by twin scale.


Some experiments were carried out to establish a protocol aiming to micropropagateHippeastrum hybridum Hort. by bulb explant. In the first experiment different combinations of hormones were tested: 3-indolacetic acid (IAA), at 0.0; 2.5 and 5.0 mg.L-1 and 6-benzilaminopurine (6-BA), at 0.0; 5.0; 10.0 and 20.0 mg.L-1. On the second experiment, the auxins - 3-indolacético acid (IAA), alfanaftalenoacetic acid (NAA) and 3-indolbutiric acid (IBA) - were tested at the concentrations 2.5 and 5.0 mg.L-1 combined with 10.0 mg.L-1 of the citocinin 6- BA. According to the statistics analysis of the results, in the first experiment was attested that only the 6-BA influenced the number of the bulblets produced. On the second experiment, it was observed that the type of auxin interfered on the quantity of generated roots. The NAA induced the formation of the largest number of roots. For the Hippeastrum mass propagation, it is important that the medium improve the number of bulblets with low quantity of roots. In this manner, the best medium obtained in these experiments contained 2.5 mg.L-1 of IAA and 10 mg.L-1 of 6-BA.

ISSN: 2447-536X

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