Cultivation period, lighting conditions and BAP concentrations on in vitro induction shoots of Dendrobium phalaenopsis Deang Suree

Yara Brito Chaim Jardim Rosa, Cryslaine Cintia Alves dos Reis, Jaqueline Clara Longo Casemiro, Jackeline Schultz Soares, José Carlos Sorgato, Camila Soares Rosa Lemes

Abstract


The technique of in vitro micropropagation allows the production of large amounts of plants. However, some aspects of the orchid’s in vitro micropropagation are still poorly studied. This study analyzed the lighting conditions, time of cultivation and BAP concentrations on in vitro budburst of Dendrobium phalaenopsis Deang Suree. Therefore, a quantitative analysis of biometric parameters of plants grown during 90 and 180 days on ½ MS medium under different lighting conditions (18.90 mol m-2 s-1, 14.85 mol m-2 s-1, 9.45 mol m-2 s-1) and concentrations of BAP (0; 0.5; 1; 1.5; 2; 2.5 e 3 mg L-1). The best results of budburst were obtained during 180 days, under white fluorescent light (18.90 mol m-2 s-1) and using 1.5±0.1 mg L-1 of BAP.

Keywords


Floriculture, cytokinin, in vitro propagation.



DOI: https://doi.org/10.14295/oh.v21i3.621

ISSN: 2447-536X

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