African violet micropropagation

Nutritive media in micropropagation are sources of essential compounds for the propagules growth. Among the nutritive media components is the group of plant growth regulators. For micropropagation of the African violet (Saintpaulia ionantha Wendl.) there are many well developed protocols. This work was carried out to compare some auxin:cytokinin ratios for African violet micropropagation and to indicate the better ratio for size and weight of plantlets from leaves explants towards the seedlings acclimatization phase. The sis media studied differ from each other by the source and the concentration of auxins and cytokinins, as follows: A, kinetin (0.2 mg.L-1) and naphthaleneacetic acid (0.2 mg.L-1); B, 6-benzyladenine (0.5 mg.L-1) and naphthaleneacetic acid (0.1 mg.L-1); C, without plant regulators; D, 6-benzyladenine (0.5 mg.L-1); E, 6-benzyladenine (0.05 mg.L-1) and naphthaleneacetic acid (0.1 mg.L-1); F , 6-benzyladenine (0.08 mg.L-1); indoleacetic acid (2.0 mg.L-1). Eleven treatments were tested, consisting of different sources and concentrations of the plant growth regulators for the three phases. During the first phase (establishment), the inclusion of plant growth regulators (auxin and cytokinin) was necessary in order to induce the shoot formation. Differences between sources and concentrations of the plant growth regulators were observed and the medium with kinetin (0.2 mg.L-1) and naphthaleeneacetic acid (0.2 mg.L-1) was more efficient than the others. At the second phase (multiplication), plants removed from the medium with kinetin and naphthaleneacetic acid of the first phase and transferred to a medium with 6-benzyladenine (0.5 mg.L -1) showed uniform development and produced the highest number of viable shoots by explants. At the third phase (rooting), the shoot from the media B, E and F, showed the higher root and shoot dry matter increasing.

 



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