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Regeneration of Gladiolus callus.


For in vitro proliferation of gladiolus (Gladiolus x grandiflorus L.) callus, buds as explants added into MS medium with 0.5 mg/L of BAP and 1.0 mg/L of ANA were utilized. For regeneration callus the concentrations of BAP (0, 0.5, 1.0, 2.0 and 4.0 mg/ L) in combination with ANA (0, 0.1 and 1.0 mg/L) in all the possible combinations making up a 5 x 3 factorial scheme were tested. Regeneration of the callus formed was better at the concentration 0.1mg/ L of ANA and absence of BAP, yielding on average 2.07 shoot with the average size of 2.27 cm. The objective of word was find a ideal concentration of growth regulators for regeneration of plants from callus of gladiolus.

ISSN: 2447-536X

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