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Evaluation of different concentrations of MS and Knudson culture media for the in vitro propagation of imperial bromeliad.


The propagation of bromeliads using tissue culture has received considerable importance. Through the plant production in large scale it is possible to attend both national and international consumers as well as programs for the reintroduction of species in their habitats especially those considered endangered. For this process, the definition of the type of culture medium and the concentration to be used is very important to determine an efficient and economical protocol for propagation of a species. The objective of this work was to evaluate the behavior of imperial bromeliad (Alcantarea imperialis) plantlets in different concentrations of MS (50 and 150% of the original concentration) and Knudson (50 and 150% of the original concentration) media using as control their original concentration (100%). All media were supplemented with 2 mg L-1 thidiazuron (TDZ), 30 g.L-1 sucrose and 7 g.L-1 agar. Number of responsive explants, shoot number per explant, shoot height and swelling were evaluated 120 days after inoculation. It was observed that the use of 100% MS medium provided the best results.

ISSN: 2447-536X

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