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Influence of biphasic tissue culture medium in the bud multiplication and on the shoot elongation in vitro of Ananas lucidus Miller.


The study was conducted at Biotechnology Laboratory of Embrapa Tropical Agroindustry in Fortaleza (Ceará), Brazil. The objective of this workwas to evaluate influence of biphasic tissue culture medium in the bud multiplication and on the shoot elongationin vitro of ornamental pineapple (Ananas lucidus Miller). It was used bud multiplication explants ofin vitro culture with 0,5 cm of height. The tissue culture medium used was MS, solid and liquid, supplemented with BAP (1 mg.L-1) and ANA (0,1 mg.L-1). The statistic delineation was completely randomized design with the following treatments: additions of 10 mL of liquid tissue culture medium per glass in periods of cultive: at 0 day, 15 days, 30 days, 15 and 30 days, 30 and 60 days, and in each 15 days for 75 days, with 14 replicated and 6 explants per plot. The evaluated parameters were: fresh weight matter and dry weight matter at 90 days of culture. In this same period the cultures of each treatments were removed for a basic tissue culture medium MS solidified with agar 0,4% (p/v) and supplemented with ANA (0,01 mg.L-1) for shoot elongation, using as explants clusters of buds with 0,5 cm of diameter. The statistic delineation was completely randomized design with 6 replicated and 6 explants per plot. There were three successive subcultives for this same new medium in each 30 days. At 90 days of culture in the shoots elongation medium were evaluated the number of shoot/ explant, shoot number percentage and percentage of shoot rooting in four amplify of height. The highest value for fresh weight matter (913,7 mg/explant) and dry weight matter (69,1 mg/explant) were obtained with addition of 10 mL in the liquid tissue culture medium at 30 days of culture. The additions of liquid tissue culture medium at 15 and 30 days of culture provided the highest mean of number of shoot (32.6 shoot/explant) at 90 days of culture in the shoot elongation medium. In all treatments the highest percentage of number of shoot and percentage of shoot rooting were observed in amplify of 1,0 cm to 2,0 cm of height.

ISSN: 2447-536X

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